What is HTG? – high throughput genomic sequences

The HTG division of DDBJ/EMBL-Bank/GenBank was created to accommodate a growing need to make unfinished genomic sequence data rapidly available to the scientific community.
The HTG division contains unfinished genome sequences generated by the high-throughput sequencing centers.
When sequences are considered to be finished level, the data will be moved from HTG to corresponding taxonomic division.

You can submit HTG data to DDBJ through Mass Submission System (MSS).

 

Notes on HTG submission

Prior to sequence data submission, get a BioProject ID for your project on the BioProject Database
Clone ID should be described in clone qualifier.
Basically, main targets of HTG division are unfinished sequences of BAC, YAC, fosmid clones.

 

Aspects of HTG on DDBJ flat file

If the sequence is considered to be finished, LOCUS line provides the division name according to taxonomic lineage; either of "HUM", "PRI", "ROD", "MAM", "VRT", "INV", "PLN" or "BCT".
If the sequence is not finished level, the division name is "HTG".
If the sequence is considered to be finished, there is no keyword in KEYWORDS.
If the sequence is not finished level, "HTG" and either of "HTGS_PHASE0", "HTGS_PHASE1" or "HTGS_PHASE2" are appeared as keywords.

HTGS_PHASE0; one-to-few pass reads of a single clone
HTGS_PHASE1; unfinished, may be unordered, unoriented contigs, with gaps.
HTGS_PHASE2; unfinished, ordered, oriented contigs, with or without gaps.

Optionally, KEYWORDS line provides some other keywords, "HTGS_DRAFT", "HTGS_ENRICHED", "HTGS_POOLED_CLONE" or "HTGS_POOLED_MULTICLONE".

 

Sample of HTG flat file

LOCUS       AP000000                1450 bp    DNA     linear   HTG 15-OCT-2008
DEFINITION  Arabidopsis thaliana DNA, chromosome 1, BAC clone: CIC5D1, ***
            SEQUENCING IN PROGRESS ***, 10 unordered pieces.
ACCESSION   AP000000
VERSION     AP000000.1
KEYWORDS    HTG; HTGS_PHASE1.
SOURCE      Arabidopsis thaliana (thale cress)
  ORGANISM  Arabidopsis thaliana
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
            Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
            rosids; malvids; Brassicales; Brassicaceae; Camelineae;
            Arabidopsis.
REFERENCE   1  (bases 1 to 423)
  AUTHORS   Mishima,H., Yamada,T. and Liu,G.Q.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2008) to the DDBJ/EMBL/GenBank databases.
            Contact:Hanako Mishima
            National Institute of Genetics, DNA Data Bank of Japan; Yata 1111,
            Mishima, Shizuoka 411-8540, Japan
REFERENCE   2
  AUTHORS   Mishima,H., Yamada,T., Park,C.S. and Liu,G.Q.
  TITLE     Arabidopsis thaliana DNA
  JOURNAL   Unpublished (2008)
FEATURES             Location/Qualifiers
     source          1..423
                     /chromosome="1"
                     /clone="CIC5D1"
                     /clone_lib="AT01 BAC"
                     /db_xref="taxon:3702"
                     /ecotype="columbia"
                     /map="between mi303 and mi259"
                     /mol_type="genomic DNA"
                     /organism="Arabidopsis thaliana"
     gap             2079..2128
                     /estimated_length=unknown
     gap             7295..7344
                     /estimated_length=unknown
     gap             15694..15743
                     /estimated_length=unknown
     gap             32780..32829
                     /estimated_length=unknown
     gap             40371..40420
                     /estimated_length=unknown
     gap             59441..59490
                     /estimated_length=unknown
     gap             79080..79129
                     /estimated_length=unknown
     gap             88074..88123
                     /estimated_length=unknown"
     gap             107128..107177
                     /estimated_length=unknown
BASE COUNT          105 a          98 c          112 g          108 t
ORIGIN
        1 attaatataa gctaaatatg tttttcaata tatattgata atagaatatc aacaatttgg
        :
        -- The rest of nucleotide sequence is omitted --
        :
//