[Caution] DDBJ terminated accepting new submission of MGA data.
In order to accept a large scale of sequence data that provide useful information for annotation of genome assemblies/sequences, the International Nucleotide Sequence Database Collaboration (INSDC; DDBJ/EMBL-Bank/GenBank) have created a new category. The name of this new category is Mass sequence for Genome Annotation (MGA). The definition of MGA data is the following.
The accession number assigned to each of MGA entries is composed of 12 letters (five alphabetical characters and seven numeric numbers). The detail of accession number is shown as follows;
MGA data are published a resource as a unit. The data consist of "Master record" and "Variable record" for a resource.
LOCUS ZZZZZ0000000 mRNA linear ROD 24-JAN-2005 DEFINITION Mus musculus 1 month adult cerebellum short transcripts tag. ACCESSION ZZZZZ0000000 VERSION ZZZZZ0000000.1 KEYWORDS MGA; CAGE (Cap Analysis Gene Expression). SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Mus. REFERENCE 1 AUTHORS Mishima,H. and Shizuoka,T. TITLE Direct Submission JOURNAL Submitted (30-NOV-2009) to the DDBJ/EMBL/GenBank databases. Contact:Hanako Mishima National Institute of Genetics, DNA Data Bank of Japan; Yata 1111, Mishima, Shizuoka 411-8540, Japan REFERENCE 2 AUTHORS Mishima,H., Shizuoka,T. and Fuji,I TITLE The gene expression analysis of short transcripts tags JOURNAL Unpublished (2010) COMMENT The CAGE (cap analysis gene expression) is based on preparation and sequencing of concatamers of DNA tags deriving from the initial 20/21 nucleotides from 5' end mRNAs. Full-length cDNAs were at first selected with the Cap-Trapper method. Then, a specific linker (Linker1, some linker contain 5 bp sequences that have 15 variations for each rna sample) containing the ClassIIs restriction enzyme site MmeI was then ligated to the single-strand cDNA and then the second strand of cDNA synthesized. (skip the rest in COMMENT field) FEATURES Location/Qualifiers source /db_xref="taxon:10090" /dev_stage="1 month adult" /mol_type="mRNA" /organism="Mus musculus" /strain="C57BL/6J" /tissue_type="cerebellum" MGA ZZZZZ0000001-ZZZZ0340780 total number of count : 856609 Header Format >[ACC#]|[submitter's identifier]|[number of sequence count]|[map]|[free text]|[db_xref1(,db_xref2,...)]| //
>ZZZZZ0000001|ABC1004AA60F1902|10|9B|lipidosis-related protein Lipidosin| GI:2385656| gactgtcttcggtgaatgca >ZZZZZ0000002|ABC1003AE78G1607|5|||| gcggaagtcggaccggtcgca >ZZZZZ0000003|ABC1003AE72P1806|6|||| gggagaccgatccgggatct >ZZZZZ0000004|ABC1003AE30G1801|91|||| gagtcgggtcggtggggctgt >ZZZZZ0000005|ABC1003AA45J1501|55|||| ggggaatctgcagcctgggc >ZZZZZ0000006|ABC1003AE67B0902|152|||| gagccgtccccgacgccgcca (skip the rest)