We at DDBJ recommend using Mass Submission System (MSS) when:
Nucleotide Sequence Submission System is an interactive application to enter all of items required for your submission on step by step basis. When the submission contents are not so many, Nucleotide Sequence Submission System is very useful, an easy procedure for your submission. However, in case of large scale submission, such as thousands of ESTs, it is more useful to send submission files directly to DDBJ, rather than Nucleotide Sequence Submission System
MSS is a procedure for large scale submission.
Before sending your data to DDBJ, please apply from MSS application form.
Then, DDBJ will introduce MSS manuals and software tools to help your submission.
For using MSS, following two files are basically required;
The text file that contains all nucleotide sequences in FASTA format.
The tab delimited text file that contains your data other than sequences, such as submitters, references and biological features.
Please apply for your submission from MSS application form.
Or, please contact to if you need preliminary consultation.
Please make samples for both of sequence and annotation files with some of your entries.
Then, please send them to DDBJ by e-mail or file transfer.
If the total size of files is more than 10 M bytes, we recommend you to use file transfer by SCP using public/private key pair.
Before sending the files, please check them with Parser tool and fix error(s) in your files if there is any error.
The test data will be validated in accordance with an international rule agreed with DDBJ/EMBL-Bank/GenBank and DDBJ rule.
We ask you to revise the files, if necessary.
We will review the data. We ask you to revise the files, if necessary
If you do not set any hold-date, your data will be released immediately.
When you set a hold-date for your data, we will release your data according to Principle of "Hold-Until-Published" data release
Based on your sequence and annotation files, your data will be processed and publicized into the DDBJ format, so called "flat file".
See also relationships between annotation file and DDBJ flat file.
Before using the file server, you have to take D-way login account.
Please visit DDBJ Submission Portal D-way to take D-way login account and to upload files.
See also an introduction for uploading files and generating key pair on D-way Submission Account Manual
You can also refer to Video Tutorial by watching the movie for each task.