The feature keys used and recommended for DDBJ submissions are as follows.
For further information of INSDC feature keys, read:
The DDBJ/EMBL/GenBank Feature Table Definition:
7.2 Appendix II: Feature keys reference.
- assembly_gap
- C_region
- CDS
- centromere
- D-loop
- D_segment
- enhancer
- exon
- gap
- intron
- J_segment
- LTR
- mat_peptide
- misc_binding
- misc_difference
- misc_feature
- misc_RNA
- misc_signal
- misc_structure
- mobile_element
- modified_base
- mRNA
- ncRNA
- operon
- oriT
- precursor_RNA
- primer_bind
- promoter
- protein_bind
- RBS
- repeat_region
- rep_origin
- rRNA
- sig_peptide
- source
- stem_loop
- telomere
- terminator
- tmRNA
- transit_peptide
- tRNA
- unsure
- V_region
- V_segment
- variation
- 3'UTR
- 5'UTR
assembly_gap
gap between two components of a CON record that is part of a genome assembly
C_region
Constant region of immunoglobulin light and heavy chains, and T-cell receptor alpha, beta, and gamma chains. Includes one or more exons depending on the particular chain.
CDS
coding sequence; sequence of nucleotides that corresponds with the sequence of amino acids in a protein (location includes stop codon). See also the page about CDS feature.
centromere
region of biological interest identified as a centromere and which has been experimentally characterized
D-loop
displacement loop; a region within mitochondrial DNA in which a short stretch of RNA is paired with one strand of DNA, displacing the original partner DNA strand in this region; also used to describe the displacement of a region of one strand of duplex DNA by a single stranded invader in the reaction catalyzed by RecA protein
D_segment
Diversity segment of immunoglobulin heavy chain, and
T-cell receptor beta chain.
enhancer
a cis-acting sequence that increases the utilization of (some) eukaryotic promoters, and can function in either orientation and in any location (upstream or downstream) relative to the promoter
exon
region of genome that codes for portion of spliced mRNA, rRNA and tRNA
gap
gap in the sequence; sequencing gap other than assembly_gap
intron
a segment of DNA that is transcribed, but removed from within the transcript by splicing together the sequences (exons) on either side of it
J_segment
Joining segment of immunoglobulin light and heavy chains, and T-cell receptor alpha, beta, and gamma chains.
LTR
long terminal repeat, a sequence directly repeated at both ends of a defined sequence, of the sort typically found in retroviruses
mat_peptide
mature peptide or protein coding sequence; coding sequence for the mature or final peptide or protein product following post-translational modification. The location does not include the stop codon (unlike the corresponding CDS)
misc_binding
site in nucleic acid which covalently or non-covalently binds another moiety that cannot be described by any other Binding key (primer_bind or protein_bind)
misc_difference
feature sequence is different from that presented in the entry and cannot be described by any other "difference" key (conflict, unsure, old_sequence, mutation, or modified_base)
Comment
The misc_difference feature should be used to describe variability that arises as a result of genetic manipulation (e.g. site directed mutagenesis).
misc_feature
region of biological interest which cannot be described by any other feature key; a new or rare feature.
misc_RNA
any transcript or RNA product that cannot be defined by other RNA keys
prim_transcript, precursor_RNA, mRNA, 5'UTR, 3'UTR, exon, CDS, sig_peptide, transit_peptide, mat_peptide, intron, polyA_site, ncRNA, rRNA and tRNA
misc_signal
any region containing a signal controlling or altering gene function or expression that cannot be described by other "Signal" keys (promoter, CAAT_signal, TATA_signal, -35_signal, -10_signal, GC_signal, RBS, polyA_signal, enhancer, attenuator, terminator, and rep_origin)
misc_structure
any secondary or tertiary nucleotide structure or conformation that cannot be described by other "Structure" keys (stem_loop and D-loop)
mobile_element
region of genome sequence containing mobile element
modified_base
the indicated nucleotide is a modified nucleotide and should be substituted for by the indicated molecule (given in the mod_base qualifier value)
mRNA
messenger RNA; includes 5'untranslated region (5'UTR), coding sequences (CDS, exon) and 3'untranslated region (3'UTR)
ncRNA
a non-protein-coding gene, other than ribosomal RNA and transfer RNA, the functional molecule of which is the RNA transcript
operon
region containing polycistronic transcript including a cluster of genes that are under the control of the same regulatory sequences/promoter and in the same biological pathway
oriT
iorigin of transfer; region of a DNA molecule where transfer is initiated during the process of conjugation or mobilization
precursor_RNA
any RNA species that is not yet the mature RNA product; may include 5' untranslated region (5'UTR), coding sequences (CDS, exon), intervening sequences (intron), and 3' untranslated region (3'UTR)
primer_bind
Non-covalent primer binding site for initiation of replication, transcription, or reverse transcription. Includes site(s) for synthetic e.g., PCR primer elements
promoter
region on a DNA molecule involved in RNA polymerase binding to initiate transcription
protein_bind
non-covalent protein binding site on nucleic acid
RBS
ribosome binding site
repeat_region
region of genome containing repeating units
rep_origin
origin of replication; starting site for duplication of nucleic acid to give two identical copies
rRNA
mature ribosomal RNA; the RNA component of the ribonucleoprotein particle (ribosome) which assembles amino acids into proteins
sig_peptide
signal peptide coding sequence; coding sequence for an N-terminal domain of a secreted protein; this domain is involved in attaching nascent polypeptide to the membrane; leader sequence
source
identifies the biological source of the specified span of the sequence. This key is mandatory. Every entry will have, as a minimum, a single source key spanning the entire sequence.
More than one source key per sequence is permissible
stem_loop
hairpin; a double-helical region formed by base-pairing between adjacent (inverted) complementary sequences in a single strand of RNA or DNA
telomere
region of biological interest identified as a telomere and which has been experimentally characterized
terminator
sequence of DNA located either at the end of the transcript that causes RNA polymerase to terminate transcription
tmRNA
transfer messenger RNA; tmRNA acts as a tRNA first, and then as an mRNA that encodes a peptide tag; the ribosome translates this mRNA region of tmRNA and attaches the encoded peptide tag to the C-terminus of the unfinished protein; this attached tag targets the protein for destruction or proteolysis;
transit_peptide
transit peptide coding sequence; coding sequence for an N-terminal domain of a nuclear-encoded organellar protein; this domain is involved in post-translational import of the protein into the organelle
tRNA
mature transfer RNA, a small RNA molecule (75-85 bases long) that mediates the translation of a nucleic acid sequence into an amino acid sequence
unsure
author is unsure of exact sequence in this region
V_region
Variable region of immunoglobulin light and heavy chains, and T-cell receptor alpha, beta, and gamma chains. Codes for the variable amino terminal portion. Can be made up from V_segments, D_segments, N_regions and J_segments.
V_segment
Variable segment of immunoglobulin light and heavy chains, and T-cell receptor alpha, beta, and gamma chains. Codes for most of the variable region (V_region) and the last few amino acids of the leader peptide
variation
a related strain contains stable mutations from the same gene (e.g., RFLPs, polymorphisms, etc.) which differ from the presented sequence at this location (and possibly others).
3'UTR
region at the 3' end of a mature transcript (following the stop codon) that is not translated into a protein.
5'UTR
region at the 5' end of a mature transcript (preceding the initiation codon) that is not translated into a protein.
