You may enter incorrect values for Location
and/or /codon_start of
CDS feature.
If the value of /codon_start is either of “2” or “3”, the location of
CDS feature should be 5’ end not complete.
See Description of Location and modify the
location with flag for “5’ end not complete”, for an example, from
“1..300” to “<1..300”.
When the CDS feature is started with an initiation codon, correct
/codon_start with “1”.