In general, see How to describe CDS feature, when termination codon is
found in the range.
You can also see Protein Coding Sequence; CDS
feature to describe CDS feature.
Following items are case study for the error.
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Did you correctly specify /codon_start qualifier to indicate reading frame of the CDS feature?
Select 1, 2 or 3, appropriately.- References:
- Offset of the frame at translation initiation by codon_start
- How to fix error message: “First codon [***] is not a start codon.” / “Final codon [***] is not a stop codon.”? How to fix error message: “Value of [ codon_start ] is not 1, but [
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Have you specify correct genetic code for /transl_table qualifier?
See followings and specify genetic code, appropriately. -
Are there really some stop codons in the range of CDS feature because of frame shift, nonsense mutation, or some other reason?
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In case of pseudogene
Click [Select Qualifier] button beside CDS and add /pseudogene qualifier. Then, you can specify /pseudogene qualifier with its controlled vocabularies.
See also b) considered pseudogene in detail. -
In cases of unsure whether it is pseudogene or not, the reason of stop codon is uncertain, or on the process of diversity increasing related to acquired immunity, describe misc_feature, not CDS feature.
See a) Putative nonsense mutation, frameshift caused by uncertain reason, or on the process of diversity increasing related to acquired immunity for IgG etc. in detail.
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In other cases.
There are some possibilities to output this error because of ribosomal slippage, RNA editing, exceptional amino acid usage, transpon insertion and so on.