The nucleotide sequence database collected and maintained by DDBJ is quarterly released online to the public and distributed in the magnetic media. We completed DDBJ Release 45 in Feb. 2001. DDBJ Release 45 consists of 11,434,113 entries, and the number of bases reached 12,207,092,905. From the present release, we include a new division, HTC (High Throughput cDNA).