WABI MAFFT（休止中）
MAFFT の job の投入
以下のパラメーターを POST します。
format, result は必須です。
result に mail を指定した場合は address も必須となります。
| パラメーター | 説明 |
|---|---|
| format | request ID を返す際の応答データの形式。 text, json, xml, bigfile, imagefile, requestfile が受け付けられますが、job投入時に意味があるのは text, json, xml の3つです。 |
| querySequence | multiple alignment 実行時に mafft に渡す配列 fasta ファイル。 |
| profile1 | Group-to-group alignment 実行時に mafft に –seed で渡す1つめの整列済み配列ファイル。 |
| profile2 | Group-to-group alignment 実行時に mafft に –seed で渡す2つめの整列済み配列ファイル。 |
| aaMatrix | multiple alignment 実行時に mafft に –aamatrix で渡すアミノ酸のカスタム weight matrix ファイル。 |
| addSequence | multiple alignment実行時にmafftに–addで渡す配列fastaファイル。 |
| addfragmentsSequence | multiple alignment実行時にmafftに–addfragmentsで渡す配列fastaファイル。 |
| addprofileProfile | multiple alignment実行時にmafftに–addprofileで渡す配列fastaファイル。 |
| addfullSequence | multiple alignment実行時にmafftに–addfullで渡す配列fastaファイル。 |
| parameters | mafft実行時のコマンドラインオプションのうち、–seed, –aamatrix, –add, –addfragments, –addprofile, –addfull 以外のものを記述する。 |
| result | 結果通知方法。www, mail のいずれか。 mail の場合、job 完了時に address に記述したメールアドレスに対して検索終了の通知が送信されます。 www の場合は何もしないので、POST 時に返された request ID を使って GET で job の状態を調べます。 |
| address | esult で mail を指定した場合に検索終了の通知を受け取るメールアドレス。 |
MAFFT の結果の取得
| パラメーター | 説明 |
|---|---|
| GET mafft/{id}?info=status | job が走っている、キューにたまっている、終わっている、存在しない、ということを返します。 |
| GET mafft/{id}?info=request | プログラム実行条件を返します。 存在しない場合は、エラーを返します。 |
| GET mafft/{id}?info=result | job が終わっていたら結果（multiple alignment, Group-to-group alignmentの結果）を返します。 終わってない・存在しない場合は、エラーを返します。 |
サンプルスクリプト
mafft-client.pl
例1：複数配列の multiple alignment
conf.json
{
"url": "http://ddbj.nig.ac.jp/wabi/mafft/",
"querySequence": "/home/hoge/cyc_aa.fasta",
"parameters": "--globalpair --maxiterate 1000",
"result": "www",
"address": ""
}
cyc_aa.fasta
>mms:mma_0447
MYRFTKTVVALLLATSGTMALAQAAYTNIGRPATAKEIAAWDIDVRPDFKGLPPGSGTVA
KGMAVWEGKCASCHGTFGESNEVFTPIVGGTTKEDIKSGHVAALSNNKQPQRTTIMKVPT
VSTLWDYINRAMPWTAPKSLTTEEVYAVTAYILNMAEILPDDFTLSDKNIAEVQQLMPNR
NGMTRNHGMWDIKGKPDVKSVACMKDCKTSTDLRSTLPEPSRNAHGNIQLQNRTFGEVRG
VDTTKPASTKPISAISADQKLAMATPAKPAAPAKVDGLALAKQYACVACHGVSNKIIGPG
FNEIAAKYKGDAAAPAALTAKIKNGSTGAWGPIPMPAQAHVKDEDIKTLVGWIIGGAK
>har:HEAR1189
MSRFTKTVVALALVATGAIACAQTAYPHIGRTATEKEIAAWDIDVRPDFKGLPKGSGTVS
KGMEVWEGKCASCHGTFGESNEVFTPIVGGTTKEDVKTGRVAALATNKQPQRTTMMKVAT
VSTLWDYINRAMPWTAPKSLTTEEVYAVTAYILNMSEVLPDDFTLSDKNIAEVQKLMPNR
NGMTQKHGMWDVKGKPDVKSVACMKDCQVSGDIRSSLPEPSRNAHGNIQEQNRSFGEVRG
VNTTVPASTTPISSATRKSVVATAATTAPAEAAAKPKALGLAKQYACIACHGVSNKIVGP
GFNEIAAKYKGDSAAATTLFDKVKNGSSGAWGPVPMPGQAHVKDEDVKTLVSWILSGAK
>pnu:Pnuc_0802
MFKLAKVAKFTLFAVTTFFAVGSVVAQNSSTHYPGIGRDATPAEVAAWDIDVRPDFKGLP
KGSGSVEKGQQLWEAKCSVCHGTFGESNEIFTPIIGGTTTDDIKTGRVASLSDRKQPQRT
TIMKVATVSSLWDYIYRAMPWNAPRSLTPDDTFALVAYLLNMAEVVPDDFVLSNTNIAEV
QKRMPNRNGMTLKHGLWNSKGTPDVHATACMTNCVQFVQIGSELPDYARNAHGNIAEQNR
QYGPFRGSDSTKPPLTKLPGASAEGLAHASETHASKKGPAELFKSENCTACHAMSTKLVG
PSVADIAAKYQGQSGALDTLMAKVKNGGSGVWGPIPMPAQSQLSDEDRKALVTWVLSGGK
>bprc:D521_0984
MFKLDKFSISLGFAALIAITAQAALAQSGSAKFPGIGRAATPAEVAAWDIDVRPDFKGLP
KGSGSVEKGQVIWEAKCASCHGTFGESNEIFTPIAGGTTKDDVKTGRVASLKDMKQPQRT
TLMKVPTVSTLWDYIYRAMPWNAPRSLTPDDTYALVAFILSLGEIVPDDFVLSDKNIAEV
KMPNRNGMTTKHGFWSVSGKPDVNGNACMHNCVPFVQIGSTLPDFARNAHENIAEQNRMY
GPYRGADTSKPPIKQLPGASGEGLAHAADTHTSAAKGPAALFKNENCSACHAPNAKLVGP
SIADIAKKYEGQSGAVDRLMAKVKNGGAGVWGSIPMPPQAQLSDDDRKTLVVWMLSGGK
>dar:Daro_3133
MSRFSKTILVLALLGASSTGFSFENFKGVGRQATPAEVKAWDIDVRPDFKGLPKGKGNVE
RGNELFEEKCASCHGSFGESNEVFTPLAGGTTKDDIKTGRVKGLSSGELPQRTTFTKVAT
ISTVFDYIQRAMPWTAPKSLKPDDVYAILAYLLNLQEIVPADFELSDRNIADVQKLLPNR
NGMTTDHGLWPGASAKKGGIGNGGIPDVKNVACMKNCKPEVQIGSTLPEYARTAHGELAD
QNRNFGAVRGTRTLGPEAAKKAADAGTLELATKSGCMACHGMKSKIVGPGYSEVVARYQG
QPDAESRLIAKVKAGGQGVWGSIPMPPNAHVKDEDLKTLVQWILAGSK
multi fasta データから global pairwise alignment で multiple alignment を行う場合のサンプル
実行されるコマンド
$ mafft --globalpair --maxiterate 1000 /home/hoge/cyc_aa.fasta > outfile
実行の様子
$ perl mafft-client.pl conf.json
Execute multiple sequence alignment.
request-ID: wabi_mafft_2013-1015-1123-50-232-114016
waiting
finished
mafft result is outputed to wabi_mafft_2013-1015-1123-50-232-114016.txt
$ cat wabi_mafft_2013-1015-1123-50-232-114016.txt
>mms:mma_0447
MYRFTKTVVALLLAT----SGTMALAQ---AAYTNIGRPATAKEIAAWDIDVRPDFKGLP
PGSGTVAKGMAVWEGKCASCHGTFGESNEVFTPIVGGTTKEDIKSGHVAALSNNKQPQRT
TIMKVPTVSTLWDYINRAMPWTAPKSLTTEEVYAVTAYILNMAEILPDDFTLSDKNIAEV
QQLMPNRNGMTRNHGMW----------DIKGKPDVKSVACMKDCKTSTDLRSTLPEPSRN
AHGNIQLQNRTFGEVRGVDTTKPASTKPISAISADQKLAMATPAKPA-APAKVDGLALAK
QYACVACHGVSNKIIGPGFNEIAAKYKGDAAAPAALTAKIKNGSTGAWGPIPMPAQAHVK
DEDIKTLVGWIIGGAK
>har:HEAR1189
MSRFTKTVVALALVA----TGAIACAQ---TAYPHIGRTATEKEIAAWDIDVRPDFKGLP
KGSGTVSKGMEVWEGKCASCHGTFGESNEVFTPIVGGTTKEDVKTGRVAALATNKQPQRT
TMMKVATVSTLWDYINRAMPWTAPKSLTTEEVYAVTAYILNMSEVLPDDFTLSDKNIAEV
QKLMPNRNGMTQKHGMW----------DVKGKPDVKSVACMKDCQVSGDIRSSLPEPSRN
AHGNIQEQNRSFGEVRGVNTTVPASTTPISSATRKSVVATAATTAPAEAAAKPKALGLAK
QYACIACHGVSNKIVGPGFNEIAAKYKGDSAAATTLFDKVKNGSSGAWGPVPMPGQAHVK
DEDVKTLVSWILSGAK
>pnu:Pnuc_0802
MFKLAKVAKFTLFAVTTFFAVGSVVAQNSSTHYPGIGRDATPAEVAAWDIDVRPDFKGLP
KGSGSVEKGQQLWEAKCSVCHGTFGESNEIFTPIIGGTTTDDIKTGRVASLSDRKQPQRT
TIMKVATVSSLWDYIYRAMPWNAPRSLTPDDTFALVAYLLNMAEVVPDDFVLSNTNIAEV
QKRMPNRNGMTLKHGLW----------NSKGTPDVHATACMTNCVQFVQIGSELPDYARN
AHGNIAEQNRQYGPFRGSDSTKP----PLTKLPGASAEGLAHASETH-AS-KKGPAELFK
SENCTACHAMSTKLVGPSVADIAAKYQGQSGALDTLMAKVKNGGSGVWGPIPMPAQSQLS
DEDRKALVTWVLSGGK
>bprc:D521_0984
MFKLDKFSISLGFAALIAITAQAALAQSGSAKFPGIGRAATPAEVAAWDIDVRPDFKGLP
KGSGSVEKGQVIWEAKCASCHGTFGESNEIFTPIAGGTTKDDVKTGRVASLKDMKQPQRT
TLMKVPTVSTLWDYIYRAMPWNAPRSLTPDDTYALVAFILSLGEIVPDDFVLSDKNIAEV
K--MPNRNGMTTKHGFW----------SVSGKPDVNGNACMHNCVPFVQIGSTLPDFARN
AHENIAEQNRMYGPYRGADTSKP----PIKQLPGASGEGLAHAADTH-TSAAKGPAALFK
NENCSACHAPNAKLVGPSIADIAKKYEGQSGAVDRLMAKVKNGGAGVWGSIPMPPQAQLS
DDDRKTLVVWMLSGGK
>dar:Daro_3133
MSRFSKTILVLALLG----ASSTGFSF---ENFKGVGRQATPAEVKAWDIDVRPDFKGLP
KGKGNVERGNELFEEKCASCHGSFGESNEVFTPLAGGTTKDDIKTGRVKGLSSGELPQRT
TFTKVATISTVFDYIQRAMPWTAPKSLKPDDVYAILAYLLNLQEIVPADFELSDRNIADV
QKLLPNRNGMTTDHGLWPGASAKKGGIGNGGIPDVKNVACMKNCKPEVQIGSTLPEYART
AHGELADQNRNFGAVRGTRTLGPEA---------------------AKKAADAGTLELAT
KSGCMACHGMKSKIVGPGYSEVVARYQGQPDAESRLIAKVKAGGQGVWGSIPMPPNAHVK
DEDLKTLVQWILAGSK
例2：2つの整列済みデータの統合（Group-to-group alignment）
conf.json
{
"url": "http://ddbj.nig.ac.jp/wabi/mafft/",
"profile1": "/home/hoge/wabi_mafft_2013-1015-1123-50-232-114016.txt",
"profile2": "/home/hoge/wabi_mafft_2013-1015-1136-16-420-058820.txt",
"parameters": "--maxiterate 1000 --clustalout",
"result": "www",
"address": ""
}
例1の結果と、もうひとつ別の multiple alignment の結果の2つを統合する場合のサンプル。
この例では出力ファイル形式を CLUSTAL 形式に指定。
実行されるコマンド
$ mafft --maxiterate 1000 --clustalout --seed /home/hoge/wabi_mafft_2013-1015-1123-50-232-114016.txt --seed /home/hoge/wabi_mafft_2013-1015-1136-16-420-058820.txt > out.txt
実行の様子
$ perl mafft-client.pl conf2.json
Execute multiple sequence alignment.
request-ID: wabi_mafft_2013-1015-1137-15-648-960788
waiting
finished
mafft result is outputed to wabi_mafft_2013-1015-1137-15-648-960788.txt
$ cat wabi_mafft_2013-1015-1137-15-648-960788.txt
CLUSTAL format alignment by MAFFT FFT-NS-i (v6.864b)
_seed_mms:mma_0 MYRFTK--TVVALLLAT----SGTMALAQ---AAYTNIGRPATAKEIAAWDIDVRPDFKG
_seed_har:HEAR1 MSRFTK--TVVALALVA----TGAIACAQ---TAYPHIGRTATEKEIAAWDIDVRPDFKG
_seed_pnu:Pnuc_ MFKLAK--VAKFTLFAVTTFFAVGSVVAQNSSTHYPGIGRDATPAEVAAWDIDVRPDFKG
_seed_bprc:D521 MFKLDK--FSISLGFAALIAITAQAALAQSGSAKFPGIGRAATPAEVAAWDIDVRPDFKG
_seed_dar:Daro_ MSRFSK--TILVLALLG----ASSTGFSF---ENFKGVGRQATPAEVKAWDIDVRPDFKG
_seed_lch:Lcho_ MSSSPKWLAAAVLALAA----AGSLAQVTA-----VGIGRAATEKEIKAWDIDVRPDFKG
_seed_reh:H16_A MSMWAELRTAAALVLAA----VSAAPAWAGTADARAALGRTATPAEVAAWDIDVRPDFQG
* : : :** ** *: **********:*
_seed_mms:mma_0 LPPGSGTVAKGMAVWEGKCASCHGTFGESNEVFTPIVGGTTKEDIKSGHVAALSNNKQPQ
_seed_har:HEAR1 LPKGSGTVSKGMEVWEGKCASCHGTFGESNEVFTPIVGGTTKEDVKTGRVAALATNKQPQ
_seed_pnu:Pnuc_ LPKGSGSVEKGQQLWEAKCSVCHGTFGESNEIFTPIIGGTTTDDIKTGRVASLSDRKQPQ
_seed_bprc:D521 LPKGSGSVEKGQVIWEAKCASCHGTFGESNEIFTPIAGGTTKDDVKTGRVASLKDMKQPQ
_seed_dar:Daro_ LPKGKGNVERGNELFEEKCASCHGSFGESNEVFTPLAGGTTKDDIKTGRVKGLSSGELPQ
_seed_lch:Lcho_ LPKGSGTVEQGMEVWEAKCAHCHGVFGESNEVFSPLVGGTTADDVKTGHVARLNDPTFPG
_seed_reh:H16_A LPRGSGTVAQGQKVWDGKCASCHGDFGESNEVFTPLVGGTTAEDIRRGRVAGMTGNQ-PY
** *.*.* :* ::: **: *** ******:*:*: **** :*:: *:* : *
_seed_mms:mma_0 RTTIMKVPTVSTLWDYINRAMPWTAPKSLTTEEVYAVTAYILNMAEILPDDFTLSDKNIA
_seed_har:HEAR1 RTTMMKVATVSTLWDYINRAMPWTAPKSLTTEEVYAVTAYILNMSEVLPDDFTLSDKNIA
_seed_pnu:Pnuc_ RTTIMKVATVSSLWDYIYRAMPWNAPRSLTPDDTFALVAYLLNMAEVVPDDFVLSNTNIA
_seed_bprc:D521 RTTLMKVPTVSTLWDYIYRAMPWNAPRSLTPDDTYALVAFILSLGEIVPDDFVLSDKNIA
_seed_dar:Daro_ RTTFTKVATISTVFDYIQRAMPWTAPKSLKPDDVYAILAYLLNLQEIVPADFELSDRNIA
_seed_lch:Lcho_ RTTLMKVATVSTLWDYINRAMPWTAPKSLKTDEVYAVTAYLLNMGDVLPAGFVLSDQTIA
_seed_reh:H16_A RTTLMKVSTVSTLWDYIHRAMPWNAPKSLSVGDVYAVTAYMLHLGEVVPADFTLSDANIA
***: **.*:*:::*** *****.**:**. :.:*: *::* : :::* .* **: .**
_seed_mms:mma_0 EVQQLMPNRNGMTRNHGMW----------DIKGKPDVKSVACMKDCKTSTDLRSTLPEPS
_seed_har:HEAR1 EVQKLMPNRNGMTQKHGMW----------DVKGKPDVKSVACMKDCQVSGDIRSSLPEPS
_seed_pnu:Pnuc_ EVQKRMPNRNGMTLKHGLW----------NSKGTPDVHATACMTNCVQFVQIGSELPDYA
_seed_bprc:D521 EVK--MPNRNGMTTKHGFW----------SVSGKPDVNGNACMHNCVPFVQIGSTLPDFA
_seed_dar:Daro_ DVQKLLPNRNGMTTDHGLWPGASAKKGGIGNGGIPDVKNVACMKNCKPEVQIGSTLPEYA
_seed_lch:Lcho_ QAQARMPNRNGMTLDHGMWPGRGL-----KTAAKPDVKVAACMSNCEAEPKVASFLPDFA
_seed_reh:H16_A EIQRRMPNRDGMTTGHGLWPGRG----------RPDTRNTACMKDCAGKVAITSSIPDYA
: : :***:*** **:* **.. *** :* : * :*: :
_seed_mms:mma_0 RNAHGNIQLQNRTFGEVRGVDTTKPASTKPISAISADQKLAMATPAKPA-APAKVDGLAL
_seed_har:HEAR1 RNAHGNIQEQNRSFGEVRGVNTTVPASTTPISSATRKSVVATAATTAPAEAAAKPKALGL
_seed_pnu:Pnuc_ RNAHGNIAEQNRQYGPFRGSDSTKP----PLTKLPGASAEGLAHASETH-AS-KKGPAEL
_seed_bprc:D521 RNAHENIAEQNRMYGPYRGADTSKP----PIKQLPGASGEGLAHAADTH-TSAAKGPAAL
_seed_dar:Daro_ RTAHGELADQNRNFGAVRGTRTLGPEA---------------------AKKAADAGTLEL
_seed_lch:Lcho_ RNAHGNLAEQTRMVGAQHGVDTTRP---------PGAAPTLAAAPVVAKATDEGAAALAL
_seed_reh:H16_A RDAHGELAQQQRSFGPVRGV----------------AAGNTVSKSAASAPSEPAAPGARL
* ** :: * * * :* *
_seed_mms:mma_0 AKQYACVACHGVSNKIIGPGFNEIAAKYKGDAAAPAALTAKIKNGSTGAWGPIPMPAQAH
_seed_har:HEAR1 AKQYACIACHGVSNKIVGPGFNEIAAKYKGDSAAATTLFDKVKNGSSGAWGPVPMPGQAH
_seed_pnu:Pnuc_ FKSENCTACHAMSTKLVGPSVADIAAKYQGQSGALDTLMAKVKNGGSGVWGPIPMPAQSQ
_seed_bprc:D521 FKNENCSACHAPNAKLVGPSIADIAKKYEGQSGAVDRLMAKVKNGGAGVWGSIPMPPQAQ
_seed_dar:Daro_ ATKSGCMACHGMKSKIVGPGYSEVVARYQGQPDAESRLIAKVKAGGQGVWGSIPMPPNAH
_seed_lch:Lcho_ AAKHTCTACHAVDAKLVGPAFREIGKKHGSRADAVAYLTGKIKSGGTGVWGAIPMPAQ-T
_seed_reh:H16_A TSQYQCMACHAMDRKLVGPSFADIAGKYKGQ-DAHGALARKVKAGGQGAWGSVPMPAQPQ
. * ***. . *::**. :: :: . * * *:* *. *.**.:*** :
_seed_mms:mma_0 VKDEDIKTLVGWIIGGA-K
_seed_har:HEAR1 VKDEDVKTLVSWILSGA-K
_seed_pnu:Pnuc_ LSDEDRKALVTWVLSGG-K
_seed_bprc:D521 LSDDDRKTLVVWMLSGG-K
_seed_dar:Daro_ VKDEDLKTLVQWILAGS-K
_seed_lch:Lcho_ LPDADAKLIANWLAAGAKK
_seed_reh:H16_A IPDSDVQAMVGWILEA--K
: * * : :. *: . *
例3：整列済みデータに新規配列を追加
conf.json
{
"url": "http://ddbj.nig.ac.jp/wabi/mafft/",
"querySequence": "/home/hoge/wabi_mafft_2013-1015-1123-50-232-114016.txt",
"addSequence": "/home/hoge/cyc_aa2.fasta",
"parameters": "--globalpair --maxiterate 1000 --clustalout",
"result": "www",
"address": ""
}
cyc_aa2.fasta
>lch:Lcho_3783
MSSSPKWLAAAVLALAAAGSLAQVTAVGIGRAATEKEIKAWDIDVRPDFKGLPKGSGTVE
QGMEVWEAKCAHCHGVFGESNEVFSPLVGGTTADDVKTGHVARLNDPTFPGRTTLMKVAT
VSTLWDYINRAMPWTAPKSLKTDEVYAVTAYLLNMGDVLPAGFVLSDQTIAQAQARMPNR
NGMTLDHGMWPGRGLKTAAKPDVKVAACMSNCEAEPKVASFLPDFARNAHGNLAEQTRMV
GAQHGVDTTRPPGAAPTLAAAPVVAKATDEGAAALALAAKHTCTACHAVDAKLVGPAFRE
IGKKHGSRADAVAYLTGKIKSGGTGVWGAIPMPAQTLPDADAKLIANWLAAGAKK
>reh:H16_A3571
MSMWAELRTAAALVLAAVSAAPAWAGTADARAALGRTATPAEVAAWDIDVRPDFQGLPRG
SGTVAQGQKVWDGKCASCHGDFGESNEVFTPLVGGTTAEDIRRGRVAGMTGNQPYRTTLM
KVSTVSTLWDYIHRAMPWNAPKSLSVGDVYAVTAYMLHLGEVVPADFTLSDANIAEIQRR
MPNRDGMTTGHGLWPGRGRPDTRNTACMKDCAGKVAITSSIPDYARDAHGELAQQQRSFG
PVRGVAAGNTVSKSAASAPSEPAAPGARLTSQYQCMACHAMDRKLVGPSFADIAGKYKGQ
DAHGALARKVKAGGQGAWGSVPMPAQPQIPDSDVQAMVGWILEAK
例1の結果に、新規の multi fasta データを追加する場合のサンプル
この例では出力ファイル形式を CLUSTAL 形式に指定。
実行されるコマンド
$ mafft --globalpair --maxiterate 1000 --clustalout --add /home/hoge/cyc_aa2.fasta /home/hoge/wabi_mafft_2013-1015-1123-50-232-114016.txt > out.txt
実行の様子
$ perl mafft-client.pl conf2.json
request-ID: wabi_mafft_2016-0704-1739-30-54-065372
waiting
finished
mafft result is outputed to wabi_mafft_2016-0704-1739-30-54-065372.txt
$ cat wabi_mafft_2016-0704-1739-30-54-065372.txt
CLUSTAL format alignment by MAFFT G-INS-1 (v7.299b)
mms:mma_0447 MYRFTKTVVALLLA-----T----SGTMALAQ---AAYTNIGRPATAKEIAAWDIDVRPD
har:HEAR1189 MSRFTKTVVALALV-----A----TGAIACAQ---TAYPHIGRTATEKEIAAWDIDVRPD
pnu:Pnuc_0802 MFKLAKVAKFTLFA-----VTTFFAVGSVVAQNSSTHYPGIGRDATPAEVAAWDIDVRPD
bprc:D521_0984 MFKLDKFSISLGFA-----ALIAITAQAALAQSGSAKFPGIGRAATPAEVAAWDIDVRPD
dar:Daro_3133 MSRFSKTILVLALL-----G----ASSTGFSF---ENFKGVGRQATPAEVKAWDIDVRPD
lch:Lcho_3783 MSSSPKWLAAAVLA-----L----AAAGSLAQ---VTAVGIGRAATEKEIKAWDIDVRPD
reh:H16_A3571 MSMWAELRTAAALVLAAVSA----APAWAGTA---DARAALGRTATPAEVAAWDIDVRPD
* : : : : :** ** *: *********
mms:mma_0447 FKGLPPGSGTVAKGMAVWEGKCASCHGTFGESNEVFTPIVGGTTKEDIKSGHVAALSNNK
har:HEAR1189 FKGLPKGSGTVSKGMEVWEGKCASCHGTFGESNEVFTPIVGGTTKEDVKTGRVAALATNK
pnu:Pnuc_0802 FKGLPKGSGSVEKGQQLWEAKCSVCHGTFGESNEIFTPIIGGTTTDDIKTGRVASLSDRK
bprc:D521_0984 FKGLPKGSGSVEKGQVIWEAKCASCHGTFGESNEIFTPIAGGTTKDDVKTGRVASLKDMK
dar:Daro_3133 FKGLPKGKGNVERGNELFEEKCASCHGSFGESNEVFTPLAGGTTKDDIKTGRVKGLSSGE
lch:Lcho_3783 FKGLPKGSGTVEQGMEVWEAKCAHCHGVFGESNEVFSPLVGGTTADDVKTGHVARLNDPT
reh:H16_A3571 FQGLPRGSGTVAQGQKVWDGKCASCHGDFGESNEVFTPLVGGTTAEDIRRGRVAGMTG-N
*:*** *.*.* :* ::: **: *** ******:*:*: **** :*:: *:* :
mms:mma_0447 QPQRTTIMKVPTVSTLWDYINRAMPWTAPKSLTTEEVYAVTAYILNMAEILPDDFTLSDK
har:HEAR1189 QPQRTTMMKVATVSTLWDYINRAMPWTAPKSLTTEEVYAVTAYILNMSEVLPDDFTLSDK
pnu:Pnuc_0802 QPQRTTIMKVATVSSLWDYIYRAMPWNAPRSLTPDDTFALVAYLLNMAEVVPDDFVLSNT
bprc:D521_0984 QPQRTTLMKVPTVSTLWDYIYRAMPWNAPRSLTPDDTYALVAFILSLGEIVPDDFVLSDK
dar:Daro_3133 LPQRTTFTKVATISTVFDYIQRAMPWTAPKSLKPDDVYAILAYLLNLQEIVPADFELSDR
lch:Lcho_3783 FPGRTTLMKVATVSTLWDYINRAMPWTAPKSLKTDEVYAVTAYLLNMGDVLPAGFVLSDQ
reh:H16_A3571 QPYRTTLMKVSTVSTLWDYIHRAMPWNAPKSLSVGDVYAVTAYMLHLGEVVPADFTLSDA
* ***: **.*:*:::*** *****.**:**. :.:*: *::* : :::* .* **:
mms:mma_0447 NIAEVQQLMPNRNGMTRNHGMW----------DIKGKPDVKSVACMKDCKTSTDLRSTLP
har:HEAR1189 NIAEVQKLMPNRNGMTQKHGMW----------DVKGKPDVKSVACMKDCQVSGDIRSSLP
pnu:Pnuc_0802 NIAEVQKRMPNRNGMTLKHGLW----------NSKGTPDVHATACMTNCVQFVQIGSELP
bprc:D521_0984 NIAEVK--MPNRNGMTTKHGFW----------SVSGKPDVNGNACMHNCVPFVQIGSTLP
dar:Daro_3133 NIADVQKLLPNRNGMTTDHGLWPGASAKKGGIGNGGIPDVKNVACMKNCKPEVQIGSTLP
lch:Lcho_3783 TIAQAQARMPNRNGMTLDHGMWPGR-----GLKTAAKPDVKVAACMSNCEAEPKVASFLP
reh:H16_A3571 NIAEIQRRMPNRDGMTTGHGLW----------PGRGRPDTRNTACMKDCAGKVAITSSIP
.**: : :***:*** **:* . **.. *** :* : * :*
mms:mma_0447 EPSRNAHGNIQLQNRTFGEVRGVDTTKPASTKPISAISADQKLAMATPAKPA-APAKVDG
har:HEAR1189 EPSRNAHGNIQEQNRSFGEVRGVNTTVPASTTPISSATRKSVVATAATTAPAEAAAKPKA
pnu:Pnuc_0802 DYARNAHGNIAEQNRQYGPFRGSDSTKP----PLTKLPGASAEGLAHASETH-AS-KKGP
bprc:D521_0984 DFARNAHENIAEQNRMYGPYRGADTSKP----PIKQLPGASGEGLAHAADTH-TSAAKGP
dar:Daro_3133 EYARTAHGELADQNRNFGAVRGTRTLGPEA---------------------AKKAADAGT
lch:Lcho_3783 DFARNAHGNLAEQTRMVGAQHGVDTTRPPGAAP----TLAAAPVVAKATDEG-AA----A
reh:H16_A3571 DYARDAHGELAQQQRSFGPVRGVAAGNTVS-----------KSAASAPSEPA-AP----G
: :* ** :: * * * :* : . .
mms:mma_0447 LALAKQYACVACHGVSNKIIGPGFNEIAAKYKGDAAAPAALTAKIKNGSTGAWGPIPMPA
har:HEAR1189 LGLAKQYACIACHGVSNKIVGPGFNEIAAKYKGDSAAATTLFDKVKNGSSGAWGPVPMPG
pnu:Pnuc_0802 AELFKSENCTACHAMSTKLVGPSVADIAAKYQGQSGALDTLMAKVKNGGSGVWGPIPMPA
bprc:D521_0984 AALFKNENCSACHAPNAKLVGPSIADIAKKYEGQSGAVDRLMAKVKNGGAGVWGSIPMPP
dar:Daro_3133 LELATKSGCMACHGMKSKIVGPGYSEVVARYQGQPDAESRLIAKVKAGGQGVWGSIPMPP
lch:Lcho_3783 LALAAKHTCTACHAVDAKLVGPAFREIGKKHGSRADAVAYLTGKIKSGGTGVWGAIPMPA
reh:H16_A3571 ARLTSQYQCMACHAMDRKLVGPSFADIAGKYKGQD-AHGALARKVKAGGQGAWGSVPMPA
* . * ***. . *::**. :: :: . * * *:* *. *.**.:***
mms:mma_0447 QAHVKDEDIKTLVGWIIGGA-K
har:HEAR1189 QAHVKDEDVKTLVSWILSGA-K
pnu:Pnuc_0802 QSQLSDEDRKALVTWVLSGG-K
bprc:D521_0984 QAQLSDDDRKTLVVWMLSGG-K
dar:Daro_3133 NAHVKDEDLKTLVQWILAGS-K
lch:Lcho_3783 QT-LPDADAKLIANWLAAGAKK
reh:H16_A3571 QPQIPDSDVQAMVGWILE-A-K
:. : * * : :. *: . *