DDBJ Annotated/Assembled Sequences
Single amplified genome
Single amplified genome (SAG) data are produced through processes of single-cell isolation, cell lysis, whole genome amplification and sequencing. Because most cells contain a single or very few genome copies, introducing even trace amounts of contaminant DNA can severely affect SAG data quality. This page describes the guideline for SAG data submission to distinguish SAG from other genomes.
Single cell
DDBJ
source feature
- For an organism name, metagenome and names with “uncultured” can not be used.
- Describe isolation source in /isolation_source. For example, “hot spring water”.
- Describe amprification method and cell numbers used in /note. For example, “one of ten single-cell amplified genomes by MDA (multiple displacement amplification)”.
BioSample
Package and attributes
- Select MISAG package.
- For an organism name, metagenome and names with “uncultured” can not be used. Use an organism name same as SAG entry.
- As a keyword, add sample_type=”single amplified genome”.
Multiple cells
DDBJ
source feature
- For an organism name, metagenome and names with “uncultured” can not be used.
- Describe isolation source in /isolation_source. For example, “hot spring water”.
- Describe amprification method and cell numbers used in /note. For example, “co-assembly of ten single-cell amplified genomes by MDA (multiple displacement amplification)”.
BioSample
Package and attributes
- Select MISAG package and create a combined sample of samples registered as “Single cell”.
- Indicate source sample accessions of the combined sample as derived_from = “SAMD00192892-SAMD00192901”