DDBJ Nucleotide Sequence Submission System (NSSS)

Submission of research data from human subjects

For all data from human subjects researches submitted to DDBJ, it is submitter’s responsibility to ensure that the dignity and the right of participant (human subject) is protected in accordance with all applicable laws, regulations and policies of submitter’s institute.
In principle, make sure to remove any direct personal identifiers of human subjects from your submissions.
Before submission, read “Submission of research data from human subjects”.

DDBJ Nucleotide Sequence Submission System

DDBJ Nucleotide Sequence Submission System, NSSS for short, is a web-based submission tool for the assembled nucleotide sequences with appropriate annotation.

Sequence Data to be submitted via MSS, NOT NSSS

NSSS cannot accept either of the following sequence data.
The nucleotide sequence data belonging to either of the following cases should be submitted via Mass Submission System (MSS).
Please note the points other than number or length of your data.

a) Either of the following categories or amounts of sequence data

EST, TSA, HTC, GSS, HTG, WGS, TLS, TPA
- See Categories for Sequence Data in detail.
Submission with long sequences, greater than 500 kb in its length
Complex submission containing many features for one sequence, more than 30 features
Submission consists of large number of sequences, greater than 100 in total

b) Regardless finished or draft level, sequence data of whole-length scale replicons

(Nuclear) genome
Organelle genome
Chromosome
Virus/Phage genome/segments
Plasmid

c) Sequence data to be described BioProject or BioSample in DBLINK

When you need to use DBLINK to link BioProject or BioSample, the following cases are included, but not limited to them.

Sequence data from metagenome analyses, environmental profilings, and so on
Sequence data of targeted genes to be linked each other
When you are planning to submit or have submitted whole genome scale data obtained from the same samples.
- Required to submit prokaryotic 16S rRNA gene for phylogenic report
- Advanced paper submission of any other targeted gene(s)/cluster region(s)

Illustrated guides

Before you start

As a first step, please read the descriptions of “Before Submission” and “Required preparation before submission”.
Previously, small scale TPA data submission was acceptable, however, currently, TPA data is not acceptable via this system.
Please submit TPA data via MSS.
See From January 2025 TPA-Exp and TPA-Inf submission types will no longer be accepted as new submissions
Trim the vector or adapter regions from the nucleotide sequences. You can check it by using VecScreen.

How to resume the submission

Save the bookmark after the clicking at each page. Then you can resume the submission from the bookmark.

Recommended internet browser

Chrome or Firefox

Updates, Questions

This system accepts only new submissions. DO NOT send update request via this system.
Regarding the update request after you have got accession number(s), see the Data Update site.
If you encounter any problem during submission, please contact us with “Submission ID” through contact form.

Start new submission
Click [Create new submission] on the next page.